Fungi associated with common bean (Phaseolus vulgaris) wilt in Costa Rica
نویسندگان
چکیده
Las pudriciones radicales limitan el rendimiento del frijol (<em>Phaseolus vulgaris</em>), y aunque en Costa Rica son frecuentes, la identidad de los hongos asociados a ellas es incierta. El objetivo esta investigación fue identificar molecularmente las marchitez dos principales zonas productoras país. Entre 2017 2020, se recolectaron 120 plantas 20 fincas regiones Huetar Norte Brunca. Se muestrearon líneas experimentales (IBC 302-29 ALS 0536-6) variedades comerciales (Brunca, Cabécar, Chánguena, Guaymí, Nambí Tayní). describieron síntomas observados campo efectuaron aislamientos agar agua rosa bengala cloranfenicol, luego cultivos puros punta hifa agua, que trasfirieron papa dextrosa para determinar morfotipos con uso claves taxonómicas. Los aislados también identificaron mediante secuenciación región ITS ADN nuclear ribosomal. calculó porcentaje frecuencia relativa morfotipos. más frecuentes fueron <em>Macrophomina phaseolina</em> (26.7%), <em>Fusarium oxysporum</em> (13.6%) <em>Athelia rolfsii</em> (5.6%). A partir aislaron comúnmente descritos esas patologías. Es necesario realizar pruebas patogenicidad confirmar agentes causales.
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ژورنال
عنوان ژورنال: Revista mexicana de fitopatología
سال: 2021
ISSN: ['0185-3309', '2007-8080']
DOI: https://doi.org/10.18781/r.mex.fit.2011-1